Hi Belinda,
I am afraid that you are going to get one of those "depends" answers.
Firstly I assume that you have treated for varroa and any supers with honey you want are off the hive?
If the colony is relatively small - i.e. a late season colony that's not much bigger than a large nuc, ( how many frames of brood do you have?) it may well be OK in a single box. However I prefer to have more food than that by having a super of food under the brood box.
See this:- http://www.norfolkbee.co.uk/beekeepers- ... reparation